Lightsheet or single plane illumination microscopy (SPIM).
The Light-Sheet Image Analysis Workshop organized by LiSIUM took place last week at Universidad Mayor in Santiago, Chile. There were 24 participants from all over Latin America—Argentina, Brazil, Chile, Colombia, Mexico, and Uruguay! Marina Cuenca, Agustin Corbat, and I (the instructors) gave lectures and practicals ranging from the basics of digital images to advanced workflows […]
The Light-Sheet Image Analysis Workshop is a five-day intensive course that will take place in Santiago, Chile, from January 5–9, 2026, designed for students and researchers who wish to gain foundational skills in the processing and analysis of light-sheet microscopy imaging data. The application deadline has been extended until August 8, 2025! The workshop is […]
I reviewed a paper last year about the embryonic development of Ceratitis capitata, the Mediterranean fruit fly (or medfly). The authors used time-lapse recordings of whole embryos made with lightsheet microscopy to create a comprehensive staging system for the embryogenesis of the species. It was one of the first reviews that I signed (disclosed my […]
In a recent email exchange, someone asked me what the colors in the video of mitotic waves mean and how did I get them. How the microscope technique results in the array of colors in the cells (purple to yellow and red). Could you briefly explain where the colors come from and how you visualised […]
My video of mitotic waves won Nikon’s 2024 Small World in Motion video competition! I’m thrilled 🎉 To learn more about the video, check out Nikon’s press release and their article for the series Masters of Microscopy. There’s also a blog post that I wrote a couple of years ago with some biological information and […]
Last year, I published a snapshot of a mitotic wave in a fruit fly embryo. Here’s the video of that same embryo going through cleavage (nuclei divisions) and gastrulation (cell movements): Mitotic waves What you see at the beginning of the movie are the cycles of synchronous nuclei divisions. They happen in waves from the […]
A mitotic wave traveling through an early #Drosophila #embryo #FlyFriday
A short video that I made about the embryonic development of the likeable Drosophila, also known as fruit fly or vinegar fly, won an honorable mention in the Small World in Motion. The details on the techniques I used and the video on its full resolution are available for download and re-use on the Wikimedia […]
Here is how a typical session of imaging embryos under Lightsheet Microscopy goes. A glimpse into my day-to-day work :) Assemble the incubation chamber: Collect and mount the embryos: Acquire a short timelapse from multiple angles: Transfer (lots of) data for image processing ;)
First day of Lightsheet Z.1 demo @Sars_Centre imaging live embryos of marine worms.