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LiSIAW 2026

The Light-Sheet Image Analysis Workshop organized by LiSIUM took place last week at Universidad Mayor in Santiago, Chile. There were 24 participants from all over Latin America—Argentina, Brazil, Chile, Colombia, Mexico, and Uruguay!

Light-Sheet Image Analysis Workshop 2026 official photo of participants, instructors, and organizers.
Official photo of the workshop by LiSIUM. Photo by Universidad Mayor.

Marina Cuenca, Agustin Corbat, and I (the instructors) gave lectures and practicals ranging from the basics of digital images to advanced workflows for visualizing, processing, and analyzing multiview lightsheet data.

20260109 191253 lisiaw organizers
Instructors and organizers :) From left to right: Marina Cuenca, Bruno Vellutini, Aníbal Vargas Ríos, Charlotte Buckley, Luz María Fuentealba, and Agustín Corbat.

I had a fantastic time. Even though I’ve been an instructor in the EMBO Light Sheet Microscopy courses, organizing this workshop from scratch and teaching lightsheet image processing in Latin America for Latin Americans was special on a different level. Especially now that I’ve just moved from Europe to start a new position in Brazil.

Materials

We have put great effort into preparing the workshop materials and making them available online. And we did so so that the participants could follow the tutorials during our practical sessions and so that anyone could access the materials afterward.

Each individual tutorial and sample dataset is now citable with a DOI from Zenodo and released under a Creative Commons license for ample reuse by the image analysis and lightsheet communities. It’s FAIR to say—they are truly Open Educational Resources.

If you are interested in image processing of lightsheet data or image analysis in general, please look at the tutorials!

Website

Snapshot of the LiSIAW website for the workshop materials built using Quarto.
Home page of the LiSIAW2026 website for hosting the workshop materials. We built it using Quarto.

Cuenca, M. B., Corbat, A. A., & Vellutini, B. C. (2026). Materials for the Light-Sheet Image Analysis Workshop 2026. Zenodo. https://doi.org/10.5281/zenodo.18247226

Link: https://bruvellu.github.io/light-sheet-image-analysis-workshop-2026

Tutorials

TitleDOI
Visualization of 2D data10.5281/zenodo.18199646
Visualization of 3D data10.5281/zenodo.18070016
Post-processing, segmentation, and labeling10.5281/zenodo.18247226
Macro programming in Fiji10.5281/zenodo.18247226
Multiview reconstruction using BigStitcher10.5281/zenodo.18090752
Object classification, dimensionality reduction, and clustering10.5281/zenodo.18247226
3D deep learning segmentation10.5281/zenodo.18187178
Tissue cartography using Blender10.5281/zenodo.18090965
Cell tracking using Mastodon10.5281/zenodo.18090897

Zenodo Community

Link: https://zenodo.org/communities/lsmunimayor

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